Genetic Plume Profiling

Characterise the potential for biological degradation of your groundwater contamination using Genetic Plume Profiling (GPP). This enables you to save on remediation costs by maximising the natural attenuation (NA) and provides a strong case towards the involved authorities. Genetic Plume Profiling can be used for all possible groundwater contaminations, such as oil, aromatics, MTBE, ETBE, TBA, chlorinated solvents and pesticides.

Step 1. Broad screening of micro-organisms present
In a first step a broad picture is formed of microbial biomass that is present at your location through ORVIdecode analyses analyses on a select number of samples. The samples are taken from different zones, for example from the source, plume and clean reference wells (to determine the natural background in the groundwater). Based on the results it is elucidated whether, and which, bacterial species are present on your site that can degrade the contaminants. Insight is also gained on the conditions that are present at the site, for example are aerobic or anaerobic biological processes dominant?

Step 2. Quantification of relevant species in a larger area
If the potential for NA is present at your location, then a greater number of samples will be analysed by ORVIdetect. in a second step. Based on the results of step 1 we will select relevant species or degradation genes that can then be quantified more accurately in a larger area. These species and/or genes can then also be monitored in time.
Every year new micro-organisms are discovered that are able to degrade contaminants. If necessary, we will develop ORVIdetect analyses for your specific situation so that you will also have the most up to date data.

Approach and results
Together with you we make a selection of the wells that are to be sampled in both steps. The required sampling material is provided by us so that you can take the samples yourself (if required this can also be done by Orvion). The groundwater samples for step 1 and 2 are taken at the same time so that only one sampling round is required.
Based on data that is available of the site (concentrations, redox conditions, pH,e tc.) and the GPP results we will consider whether natural attenuation is feasible for your site. The Genetic Plume Profiling results are visualised in maps. The turnaround time for GPP is approximately six weeks.

Contact Paul Appeldoorn to discuss the possibilities.

Paul Appeldoorn is a Civil Engineer. His ambition is to solve problems. New biotechnologies make it possible to look at soil remediation, water treatment or industrial processes with fresh eyes. But the technology only works if the problem is dissected and there is a clear financial advantage for the customer. Keep questioning until the core of the problem has been achieved and be honest about what works and what does not work.

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