Genetic Plume Profiling

The most direct way to demonstrate degradation of contaminants in groundwater is to measure whether the organisms that do so are present. Making optimum use of natural degradation leads to savings in remediation costs and is an integral part of risk-based management.

Genetic Plume Profiling is a method that provides insight into all microbiological information from ORVIdetect qPCR and ORVIdecode NGS analyzes in a flow path of contamination. The result is an interactive file with information about the organisms, the contamination and soil conditions (such as oxygen, nitrate, sulphate, pH). With the interactive file, the desired information can be filtered from the large amount of data, a few gigabytes per water sample.

An example of this is a study into the natural degradation of a naphthalene contamination at a location in Hilversum. A location managed by Bodemzorg. Peter Assenberg from Bodemzorg, part of Afvalzorg:

”Bodemzorg has many years of experience with the monitoring of soil remediation and dumping sites. We wanted a clear picture of the natural degradation potential on the site before the start of the monitoring.

By not allowing ourselves to be limited in the number of bacterial species to be measured, we have proof that there is natural attenuation of naphthalene occurring. Even in the source zone and at a depth of forty meters. “

Orvion uses molecular detection techniques to measure DNA and/or RNA in groundwater. The DNA contains genetic information about “which bacterial species are present?” And “what characteristic do the organisms have?” RNA data answers the question “are the organisms active?”.

Because of worldwide scientific research, more and more is known about contaminant-degrading organisms. For example, more than 75 organisms and genes are known that are related to degradation of BTEX. A range of degrading organisms and genes is also known for other contaminants, such as solvents, fuel additives and pesticides. And that number is increasing every year.

Please contact Paul Appeldoorn for further information

Paul Appeldoorn is a Civil Engineer. His ambition is to solve problems. New biotechnologies make it possible to look at soil remediation, water treatment or industrial processes with fresh eyes. But the technology only works if the problem is dissected and there is a clear financial advantage for the customer. Keep questioning until the core of the problem has been achieved and be honest about what works and what does not work.

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