ORVIdetect – qPCR

We offer quantitative ORVIdetect analyses for the measurement of specific types and properties of bacteria. These analyses use qPCR technology to determine the number of cells in environmental samples. The table at the end of the page provides an overview of available analyses, our standard turnaround time is five business days.

Procedure
We will send you the correct sampling material so that you can take samples yourself (or let them be taken). The bottles contain a preservative so that the samples are fixed immediately and do not have to be cooled after sampling or during transport. A sample registration form is included for fast and easy handling of the samples by our laboratory. After receipt of the samples by Orvion you will be sent the analysis results digitally within five business days.
Analytical technique
Our bacteriological counts use the qPCR technique whereby microorganisms are detected and quantified based on their genetic material (DNA or RNA). More information about our qPCR analysis procedure can be found here vinden.
Designing new analyses
Orvion designs and validates new ORVIdetect analyses in-house and for this purpose we have developed “primer design software”. If your analysis is not on the list, please contact us to discuss the possibilities.

Aleida de Vos is a molecular microbiologist. The development and implementation of biological solutions is what motivates her. The method and approach of Orvion are often new to our customers, so it is important to involve people in the process and to enthuse them for the opportunities that lie ahead. No problem is the same and that is why we always look for the most appropriate solution.

Available analyses
Universal
Detection of Goal Link
BACT Universal bacteria Broad screening of bacterial biomass Project
ARC Universal Archaea Broad screening of archaea in biomass
Degradation of chlorinated contaminants
Detection of Target Link
DEHC PCE, TCE, cisDCE and VC Dehalococcoides spp. dechlorering.nl
DEHB 1,1,1-TCA, 1,1-DCA chloroform and DCM Dehalobacter spp. dechlorering.nl
DHBM DCM Dehalobacterium
DHGM 1,2,3-TCP, 1,2-DCP, 1,2-DCA, 1,1,2-TCA en 1,1,2,2-TeCA Dehalogenimonas
GBLY TCE, pure product seeker Geobacter lovleyi (Pelobacter propionicus included)
TCEA specific conversion of TCE to DCE Trichloroethene reductive dehalogenase gene(tceA) dechlorering.nl
VCRD specific conversion of Vinylchloride (VC) to ethene vinyl chloride reductive dehalogenase(vcrA & bvcA) dechlorering.nl
DCCF 1,1,1-trichloroethane (TCA) chloroform & 1,2-dichloroethane (DCA) cfrA & dcrA(respectively)
CERA cerA (from dehalogenimonas) Anaerobic VC degradation
ETNC VC Micro-aerofilic to ethene alkene monooxygenase (etnC)
ETNE VC Micro-aerofilic to ethene epoxyalkane coenzyme M transferase (etnE)
Water treatment
Detection of Goal Link
ORVIdetect MIC Microtrix parvicella Filamentous bacterium, reduced settling
ORVIdetect FeOX Sideroxydans sp. Iron oxidation, Fe(II) to Fe(III)
ORVIdetect GEO Geobacter (includes Pelobacter propionicus) Iron reducer, Fe(III) to Fe(II)
ORVIdetect GAL Gallionella sp. Iron oxidzing bacterium
Nitrogen cycle
Detection of Goal Link
ORVIdetect NSO1 Nitrosomonas (Is79A4 strain) Nitrificatie (ammonium oxidation), conversion of ammonium (NH4) to nitrite (NO2)
ORVIdetect NSO2 Nitrosomonas Ammonium oxidizing bacteria
ORVIdetect NSP1 Nitrospira Ammonium oxidizing bacteria
ORVIdetect NSP2 Nitrospira (defluvii & moscoviensis) Nitrite oxidizing bacteria
ORVIdetect NOSZ nosZ Denitrifying bacteria
ORVIdetect KUEN candidatus Kuenenia sp. ammonium oxidation (anammox)
ORVIdetect NITO Nitrotoga Nitrite oxidizing bacteria
Pathogens
Detection of Goal Link
ORVIdetect ECOLI Escherichia coli Faecal contamination, potential pathogen, indicator organism Article ViaWater
ORVIdetect SHIG Shigella spp. Water-related pathogen (shigellose) Article ViaWater
ORVIdetect SALM Salmonella spp. Water-related pathogen (diarrhea) Article ViaWater
ORVIdetect LEPT Leptospira spp. Potential water-related pathogen (leptospirose / Weil's disease) Article ViaWater
ORVIdetect HELI Helicobacter pylori Potential water-related pathogen (gastritis) Article ViaWater
ORVIdetect LEGI Legionella pneumophila Water-related pathogen (legionellose)
ORVIdetect CAMP Campylobacter spp. Pathogen
ORVIdetect MYCO Mycobacterium Pathogen
BTEXN
Detection of Goal Link
ORVIdetect ABCA abcA (Benzene carboxylase) Anaerobic benzene degradation Article BTEX afbraak
ORVIdetect BSSA bssA (Benzylsuccinate synthase) Anaerobic degradation of Toluene and Xylenes Article BTEX afbraak
ORVIdetect EBDA ebdA (Ethylbenzene dehydrogenase) Anaerobic degradation Ethylbenzene Article BTEX afbraak
ORVIdetect BCRC bcrC (Benzoyl-CoA reductase) Anaerobic degradation of Benzoyl-COA (intemediaary anaerobic degradation of aromatics) Article BTEX afbraak
ORVIdetect BZDN bzdN (Benzoyl-CoA reductase) Anaerobic degradation of Benzoyl-COA (intemediaary anaerobic degradation of aromatics) Artikel BTEX afbraak
ORVIdetect NCRC ncrC (2-naphthoyl-CoA reductase) Anaerobic degradation of naphthoyl-CoA intermediary anaerobic degradation of Naphthalene) Artikel BTEX afbraak
ORVIdetect THAU Thauera aromatica en chlorobenzoica Anaerobic degradation of toluene and xylene
ORVIdetect BAMA bamA, (oah), badI, bzdY Anaerobic degradation of BTEX
ORVIdetect BAMB Oxoacyl-CoA hydrolase (bamB) Anaerobic degradation of BTEX
ORVIdetect RCB Dechloromonas aromatica RCB Anaerobic degradation of BTEX
Fuel additives
Detection of Goal Link
ORVIdetect MDPJ mdpJ (tert-butyl alcoholhydroxylase) Aerobic TBA degradation Factsheet MET
ORVIdetect ICMA icmA (tert-butyl alcoholhydroxylase) Aerobic MTBE/ETBE-Degradation Factsheet MET
ORVIdetect VARP Variovorax paradoxus (asfA) Specific species associated with MTBE & ETBE degradation Factsheet MET
ORVIdetect ETHB cytochrome P450 monooxygenase (ethB) Specific species associated with MTBE & ETBE degradation Factsheet MET
eDNA Ecologie
Detection of Goal Link
ORVIdetect KMK Cobitis taenia Spined loach
ORVIdetect GMK Misgurnus fossilis weatherfish Artikel AD
ORVIdetect RARK Procambarus clarkii Freshwater crayfish
ORVIdetect MUSK Ondatra zibethicus Muskrat
ORVIdetect KAMS Triturus cristatus The great crested newt
ORVIdetect MOEC Microtus oeconomus Tundra vol
ORVIdetect DKN Asian knotweed Invasive usury Artikel Duizendknoop
Pesticiden
Detection of Goal Link
ORVIdetect PSEU Pseudomonas (oprL) degradation of Mecoprop
ORVIdetect RDPA (R)-enantiomer-specific 2,4-dichlorophenoxypropionate dioxygenase (rDPA) degradation of Mecoprop
ORVIdetect SDPA (S)-enantiomer-specific 2,4-dichlorophenoxypropionate dioxygenase (sDPA) degradation of Mecoprop

ORVIdetect analyses are intended solely for research purposes, not for diagnostic purposes.

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